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A COFRADIC Protocol To Study Protein Ubiquitination

05/12/2014 13:45

Elisabeth Stes, Mathias Laga, Alan Walton, Noortje Samyn, Evy Timmerman, Ive De Smet, Sofie Goormachtig and Kris Gevaert Abstract: Here, we apply the COmbined FRActional DIagonal Chromatography (COFRADIC) technology to enrich for ubiquitinated peptides and to identify sites of ubiquitination...

Resistance to phytopathogens e tutti quanti: placing plant quantitative disease resistance on the map

05/06/2014 00:00

Fabrice Roux, Derry Voisin, Thomas Badet, Claudine Balagué, Xavier Barlet, Carine Huard-Chauveau, Dominique Roby and Sylvain Raffaele Abstract: Plant disease resistance can be seen as a process of a dual nature: both qualitative and quantitative. The nature of non-self molecules perceived by...

Distinct functional roles for the SLX4 ubiquitin-binding UBZ domains mutated in Fanconi anemia

05/02/2014 00:00

Lachaud C, Castor D, Hain K, Muñoz I, Wilson J, Macartney TJ, Schindler D, Rouse J. Abstract: Defects in SLX4, a scaffold for DNA repair nucleases, cause Fanconi anemia due to defective repair of inter-strand DNA crosslinks (ICLs). Some FA patients have an SLX4 deletion removing two tandem...

Nouvelles acquisitions sur le caractère poivré des vins rouges

05/01/2014 00:00

Olivier Geffroy, Thierry Dufourcq, Dorian Carcenac, Tracey Siebert, Markus Herderich and Eric Serrano Summary: La rotundone (Figure 1) est un composé aromatique très odorant responsable d’intenses notes poivrées dans les vins. Elle a été mise en évidence pour la première fois en 2008 dans...

Transcriptional regulators of legume-rhizobia symbiosis: Nuclear Factors Ys and GRAS are two for tango

04/15/2014 00:00

Carolina Rípodas, Joaquín Clua, Marina Battaglia, Maël Baudin, Andreas Niebel, María Eugenia Zanetti and Flavio Blanco Abstract: Transcription factors are DNA binding proteins that regulate gene expression. The nitrogen fixing symbiosis established between legume plants and soil bacteria is...

CYP77A19 and CYP77A20 characterized from Solanum tuberosum oxidize fatty acids in vitro and partially restore the wild phenotype in an Arabidopsis thaliana cutin mutant

04/09/2014 00:00

Grausem B, Widemann E, Verdier G, Nosbüsch D, Aubert Y, Beisson F, Schreiber L, Franke R, Pinot F. Abstract: Cutin and suberin represent lipophilic polymers forming plant/environment interfaces in leaves and roots. Despite recent progress in Arabidopsis, there is still a lack on information...

Exploring Picea glauca aquaporins in the context of needle water uptake and xylem refilling

04/07/2014 00:00

Joan Laur and Uwe G. Hacke Abstract: Conifer needles have been reported to absorb water under certain conditions. Radial water movement across needle tissues is likely influenced by aquaporin (AQP) water channels. Foliar water uptake and AQP localization in Picea glauca needles...

Molecular analysis reveals high compartmentalization in aphid–primary parasitoid networks and low parasitoid sharing between crop and noncrop habitats

03/26/2014 00:00

Stephane A. P. Derocles, Anne Le Ralec, Mathilde M. Besson, Marion Maret, Alan Walton, Darren M. Evans and Manuel Plantegenest Abstract: The ecosystem service of insect pest regulation by natural enemies, such as primary parasitoids, may be enhanced by the presence of uncultivated,...

E3 ubiquitin-ligases and their target proteins during the regulation of plant innate immunity

02/13/2014 00:00

Vincent Duplan and Susana Rivas Abstract: Reversible protein ubiquitination plays a crucial role during the regulation of plant immune signaling. E3 ubiquitin (Ub)-ligase enzymes, which are classified into different families depending on their structural and functional features, confer the...

CYCLOPS, A DNA-Binding Transcriptional Activator, Orchestrates Symbiotic Root Nodule Development

02/12/2014 00:00

Sylvia Singh, Katja Katzer, Jayne Lambert, Marion Cerri and Martin Parniske Abstract: Nuclear calcium oscillations are a hallmark of symbiotically stimulated plant root cells. Activation of the central nuclear decoder, calcium- and calmodulin-dependent kinase (CCaMK), triggers the entire...

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